mertk antibody

Recombinant human MERTK (mutated A708S) protein (ab167957) Results of validation by standard or enhanced validation based on assessment of antibody performance in 44 normal tissues. Publishing research using ab95925? For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Proteomic analysis of mitochondria in respiratory epithelial cells infected with human respiratory syncytial virus and functional implications for virus and cell biology. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As. MERTK is upregulated in malignant cells, including leukemia, lymphoma and colorectal cancer. Primary - For the independent antibodies validation the evaluation is based on comparison of the staining of two (or more) independent antibodies directed towards independent epitopes on the protein. It interacts with two ligands, gas6 (growth arrest-specific 6) and protein-S. Mutation in this gene, rs4374383 G>A, regulates the severity of fibrosis in patients with non-alcoholic fatty liver disease. A yellow triangle on the bar indicates a <100% sequence identity to the protein target. Western blot standard protocol optimized forKeywords: Catalytic combustion detector, Electrophoresis, Gene expression, Sample preparations, Western blot, Prestige Antigens and the corresponding Prestige Antibodies are recommended to be used according to the standard blocking protocol described below.Keywords: Immunocytochemistry, Immunohistochemistry, Immunostaining, Titrations, Western blot, Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot. 38:D142-D148 (2010) UNC569-induced Morphological Changes in Pigment Epithelia and Photoreceptor Cells in the Retina through MerTK Inhibition in Mice. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. For the siRNA validation, a box plot of the results is shown. Purified anti-MERTK (Mer) Antibody - MERTK is a receptor tyrosine kinase of the TAM (Tyro3, Axl, MERTK) family, which preferentially is expressed by macrophages, dendritic cells, and natural killer cells. The result is then scored Enhanced, Supported, Approved, or Uncertain. Contact All assays through which the antibody has been validated. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. In some cases, a COA may not be available on-line. Anti-MERTK antibody produced in rabbit has been used in immunohistochemistry. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. For orthogonal validation, representative images of high and low expression are shown. Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Unfortunately, one or more of the products below are unavailable in your country/region. | Privacy. Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee. For the GFP validation the signal overlap between the antibody staining and the GFP-tagged protein is evaluated. In case of filovirus infection, seems to function as a cell entry factor. Standard validation is based on concordance with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways:• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.• Protein array of 364 human recombinant protein fragments.• Validated for multiple commonly used applications such as IHC (Immunohistochemistry), IF (Immunofluorescence), and WB (Western Blot). Store at +4°C short term (1-2 weeks). Partly consistent with extensive gene/protein characterization data. Anti-C-mer proto-oncogene tyrosine kinase, Nucleic Acids Res. in the following tested applications. At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. Physical form Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. The staining of an antibody is evaluated by Western Blot through analysis of samples from different cell lysates. . The curve in blue displays the predicted antigenicity i.e. IHC-P, WB, ELISA, IP, Synthetic peptide corresponding to Mouse MERTK aa 900 to the C-terminus conjugated to keyhole limpet haemocyanin. All rights reserved. Shipped at 4°C. The protein is characterized with a conserved intracellular kinase domain and an extracellular adhesion molecule-like domain. If you continue, we'll assume that you are happy to receive all cookies. Immunocytochemistry is used for validating antibody reliability by assessing staining pattern in cell lines. to you upon submission of this form. Standard validation results in scores Supported, Approved or Uncertain. K9. Western blot protocols. Tyr protein kinase family. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406. Your browser does not have JavaScript enabled and some parts of this website will not work without it. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406. Datasheets and documents. ELSALYS BIOTECH has already developed a series of antibody candidates against Tyro3 and MerTK which are currently under evaluation in partnership with academic experts in in vitro models. All Rights Reserved. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. Antigen retrieval is a method used to restore/retrieve the epitope (antibody bidning region) of the target protein, cross-linked, and thus masked, during tissue preserving fixative treatment of the tissues. Please let us know so that we can cite the reference in this datasheet. Need larger quantities for your development, manufacturing or research applications? Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more. Please note: All products are "FOR RESEARCH USE ONLY. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Site Use Terms Western Blot is used for quality control of the polyclonal antibodies generated in the project. MER proto-oncogene, tyrosine kinase (MERTK) is mainly involved in the starting of efferocytosis, which is the removal of dying cells by phagocytosis. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. © 2020  Merck KGaA, Darmstadt, Germany and/or its affiliates. Upregulation of MERTK seems to also promote the survival of certain cancer cells, such as t(1;19)-positive acute lymphoblastic leukemias (ALL). If you find a lot number with a filling-code such as Goat Anti-Rabbit IgG H&L (HRP) (ab205718) Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody (ab95925), Western blot - Anti-MERTK antibody (ab95925). Enhanced validation results in the score Enhanced and includes two methods: Orthogonal validation and Independent antibody validation. Below the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). MerTK is often expressed on malignant cells and may be implicated in immune evasion. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. HPA036196 - Anti-MERTK antibody produced in rabbit, Pricing & availability is not currently available. An image representative of the antibody staining pattern is shown. Detects a band of approximately 150, 90 kDa (predicted molecular weight: 110 kDa). Antibody information for antibodies HPA036196 and HPA075622 used in analysis of ENSG00000153208 / MERTK (c-Eyk, mer, RP38, Tyro12) A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases. The membrane was then blocked for an hour using 3% Milk before being incubated with ab95925 overnight at 4°C. Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility. ICC/IF image of ab95925 stained MEF1 cells. Anti-RP38, Human Protein Atlas Number HPA036196    Human Protein Atlas - View RNA & protein expression data of this target. MerTK can be used to help discriminate macrophages from dendritic cells. Questions? For antibodies with unreliable result a revalidation using an over-expression lysate is performed. Belongs to the protein kinase superfamily. following the words 'Lot' or 'Batch'. A protein array containing 384 different antigens including the antibody target is used to analyse antibody specificity. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy, Prestige Antibodies® have been used for subcellular localization studies by immunocytochemistry (ICC) - immunofluorescence (IF) using the protocol described below.